Hydrogen Peroxide Measurement Can Be Used to Monitor Plant Oxidative Stress Rapidly Using Modified Ferrous Oxidation Xylenol Orange and Titanium Sulfate Assay Correlation

Various methodologies, sensitivities, and types of interference affect the quantification plant hydrogen peroxide (H2O2) concentration. Modified ferrous oxidation xylenol orange (eFOX) assay titanium sulfate (Ti(SO4)2 are relatively accessible methods. However, their correlation is unknown, for example whether we can get same results different species in environments. Leaf samples Ambrosia trifida, Solidago altissima, Artemisia princeps, Sicyos angulatus were collected from a riparian vegetation zone on sunny days. The H2O2 concentration leaves was evaluated two groups. Nonfrozen leaf prepared analysis soon after arriving at laboratory, frozen stored −80 °C 25 days afterwards. eFOX measure even lower fluctuations than Ti(SO4)2 assay. A substantial observed between nonfrozen (r = 0.879, p < 0.001) assays 0.837, 0.001). Sample weight did not quantification. Each showed conditions (Ambrosia trifida 0.767, 0.001), altissima 0.583, princeps 0.672, 0.828, 0.001)). Therefore, both methods be utilized easily rapidly to quantify oxidative stress using H2O2.